It is necessary to execute a prior soak (1 hour) for cysts in clean freshwater before hatching. 1. Hatching tank: A conical bottom tank works best for hatching. 2. Solution Salinity:10-35 PPT is acceptable 3. PH: optimal 8-8.5 PH. 4. Egg Density: less than 2.5g of cysts per litre of solution. 5. Temperature: optimal temperature is 28°C-30°C (for 24-28 hours) 6. Illumination: The recommended illumination is 2000 lux (no less than 1500 lux for 24 hours) 7. Aeration: Maintain strong continuous aeration to the bottom of container through airstones throughout hatching period, oxygen saturation 2-3 mg per litre of solution. 8. Hatching time: 24 hours
